Synergistic antibacterial effects of carvacrol and ?-polylysine

This study aimed to evaluate the antimicrobial efficacy of combination ?-polylysine (?-PL) and carvacrol (Car) against foodborne pathogens, Escherichia coli Staphylococcus aureus. The minimum inhibitory concentrations (MICs) ?-PL E. S. aureus were 25 ?g/mL (320 ?g/mL) 12.5 ?g/mL), respectively. Checkerboard assays showed that Car exerted synergistic effects with fraction concentration index (FICI) 0.375 0.5, It demonstrated significantly inhibited growth two strains compared single treatment. Furthermore, mode action (6.25 or (80 in inhibiting was researched by assessing their changes regard cellular membrane integrity, permeability, respiratory activity, structure. A increased damage cell membranes permeability led release 260 nm absorbing materials, decreased respiratory-chain dehydrogenase activity treatment alone. These results could be used as a new promising naturally sourced food preservative.